category: Research

Soil microbial diversity and spatiality across peatland vegetation mosaics under restoration in the southern Pennines, UK

Impacts of degraded blanket bog restoration on culturable and non-culturable soil bacterial and fungal biodiversity and spatiality were assessed. Peat sampled from unvegetated peat areas and from 3 restored vegetation classes, remnant vegetation and grass dominated gullies were subjected to culturable microbial enumeration and rDNA based community profiling and pyrosequencing. …

Impacts of peatland restoration on the identity and distribution of soil microbial communities in vegetation mosaics

Diversity and functioning of soil microbial drivers in peatland ecosystems remain poorly investigated. We report on culture-dependent and -independent characterisation of soil bacterial and fungal communities and primary geospatial relationships identified in bare peat and vegetation mosaics that have resulted from active restoration of degraded peatland in the southern Pennines.

Long-term partial nitrification in an intermittently aerated sequencing batch reactor (SBR) treating ammonium-rich wastewater under controlled oxygen-limited conditions

Highlights Development of a novel technology for partial nitrification at ambient temperature. First time to use online NH4-–N measurement as a control parameter for bioreactors. RT-PCR analysis shows the population size of AOB was up to 10% of total bacteria. Intermittent aeration and oxygen-limited conditions sustained partial nitrification. The bioreactor …

Dynamic changes in microbial community structure and function in phenol-degrading microcosms inoculated with cells from a contaminated aquifer

Contamination of aquifers by organic pollutants threatens groundwater supplies and the environment. In situ biodegradation of organic pollutants by microbial communities is important for the remediation of contaminated sites, but our understanding of the relationship between microbial development and pollutant biodegradation is poor. A particular challenge is understanding the in …

Quantification of biofilm and planktonic communities

Biofilms are difficult to quantify especially when microscopic observations are impractical. However it is important to quantify attached cell numbers to complement planktonic cell counts in order to achieve a holistic understanding of microbial processes. Traditional counting methods, including microscopic techniques and serial dilution plating, generally require biofilms to be …

Aggregative behaviour of bacteria isolated from canine dental plaque

Interbacterial adhesion of bacteria isolated from canine dental plaque was assessed by performing a visual coaggregation assay. Using conditions mimicking those likely to be encountered in vivo, the entire cultivable plaque microbiota from a single dog was assessed, and eight (6.7%) unique coaggregation interactions were detected for 120 crosses. Transmission …

Cultivable Oral Microbiota of Domestic Dogs

Abstract Bacteria were isolated from the dental plaques of nine dogs and a sample of pooled saliva from five other dogs and were then identified by comparative 16S rRNA gene sequencing. Among 339 isolates, 84 different phylotypes belonging to 37 genera were identified. Approximately half of the phylotypes were identified …

Bacterial Biofilm Development on Hydroxyapatite-Coated Glass

Glass plates are frequently used as the substratum in flow cell experiments to allow continuous non-destructive observations of biofilm development via microscopy. The aim of this study was to evaluate hydroxyapatite-coated glass as a substratum for flow cell experiments, in comparison to plain glass, for modelling primary colonization of the …