Quantification of biofilm and planktonic communities

Biofilms are difficult to quantify especially when microscopic observations are impractical. However it is important to quantify attached cell numbers to complement planktonic cell counts in order to achieve a holistic understanding of microbial processes. Traditional counting methods, including microscopic techniques and serial dilution plating, generally require biofilms to be …

Aggregative behaviour of bacteria isolated from canine dental plaque

Interbacterial adhesion of bacteria isolated from canine dental plaque was assessed by performing a visual coaggregation assay. Using conditions mimicking those likely to be encountered in vivo, the entire cultivable plaque microbiota from a single dog was assessed, and eight (6.7%) unique coaggregation interactions were detected for 120 crosses. Transmission …

Cultivable Oral Microbiota of Domestic Dogs

Abstract Bacteria were isolated from the dental plaques of nine dogs and a sample of pooled saliva from five other dogs and were then identified by comparative 16S rRNA gene sequencing. Among 339 isolates, 84 different phylotypes belonging to 37 genera were identified. Approximately half of the phylotypes were identified …

Bacterial Biofilm Development on Hydroxyapatite-Coated Glass

Glass plates are frequently used as the substratum in flow cell experiments to allow continuous non-destructive observations of biofilm development via microscopy. The aim of this study was to evaluate hydroxyapatite-coated glass as a substratum for flow cell experiments, in comparison to plain glass, for modelling primary colonization of the …