Bacterial Biofilm Development on Hydroxyapatite-Coated Glass

Glass plates are frequently used as the substratum in flow cell experiments to allow continuous non-destructive observations of biofilm development via microscopy. The aim of this study was to evaluate hydroxyapatite-coated glass as a substratum for flow cell experiments, in comparison to plain glass, for modelling primary colonization of the …

Prevalence of Csh-like fibrillar surface proteins among mitis group oral streptococci

The prevalence of Csh-like fibrillar surface proteins among oral streptococci was investigated by ELISA and by immunoelectron microscopy using antiserum raised to recombinant fragments of CshA of Streptococcus gordonii DL1. The majority of S. gordonii, Streptococcus sanguis and Streptococcus oralis strains tested elaborated short (ca. 50-80 nm long) surface fibrils …

Distribution of CshA Fibrils amongst Mitis Group Oral Streptococci

Many oral streptococci express large surface structures (fibrils and fimbriae) that have been implicated in adhesion of bacteria to oral surfaces. Streptococcus gordonii DL1 short peritrichous fibrils (61 nm long) are composed of cell-wall-anchored CshA adhesin (259 kDa). ELISA studies have previously shown that proteins immunologically related to CshA are …