Glass plates are frequently used as the substratum in flow cell experiments to allow continuous non-destructive observations of biofilm development via microscopy. The aim of this study was to evaluate hydroxyapatite-coated glass as a substratum for flow cell experiments, in comparison to plain glass, for modelling primary colonization of the …
2 days at University of Sheffield, May 2005.
University of Sheffield (Cell-Mineral interface).
Aim Evaluate hydroxyapatite-coated glass as an alternative substratum for modeling primary colonisation of the tooth surface by Streptococcus sanguis.
UCL, London. 18 March 2004
CEQ 8000 sequencer training at Beckman Coulter, High Wycombe. 26-27 Jan 2004.
UCL, London. 17-19 September 2003.
The prevalence of Csh-like fibrillar surface proteins among oral streptococci was investigated by ELISA and by immunoelectron microscopy using antiserum raised to recombinant fragments of CshA of Streptococcus gordonii DL1. The majority of S. gordonii, Streptococcus sanguis and Streptococcus oralis strains tested elaborated short (ca. 50-80 nm long) surface fibrils …
Many oral streptococci express large surface structures (fibrils and fimbriae) that have been implicated in adhesion of bacteria to oral surfaces. Streptococcus gordonii DL1 short peritrichous fibrils (61 nm long) are composed of cell-wall-anchored CshA adhesin (259 kDa). ELISA studies have previously shown that proteins immunologically related to CshA are …
Faculty of Science and Engineering, The University of Manchester. Industrial experience (sandwich placement)Quorn Plant Quality Laboratory, Billingham. ProjectDetection of CshA Polypeptide on the Cell Surface of Oral Streptococci (supervised by Dr Pauline Handley)